Antibacterial and Antifungal Properties of Citronella oil Against Streptococcus mutans and Candida albicans by In Vitro Study


  • Vinna Kurniwati Sugiaman Faculty of Densitry Universitas Kristen Maranatha Bandung
  • Wahyu Widowati Faculty of Medicine Universitas Kristen Maranatha Bandung
  • Hanna Sari Widya Kusuma Widya Kusuma Aretha Medika Utama Biomolecular and Biomedical Research Center Bandung
  • Nindia Salsabila Aretha Medika Utama Biomolecular and Biomedical Research Center Bandung
  • Rizal Rizal Biomedical Engineering Department of Electrical Engineering Faculty of Engineering Universitas Indonesia Jakarta



Antimicrobial, Candida albicans, Minimum Bactericidal Content, Minimum Inhibitory Concentration, Streptococcus mutans


Streptococcus mutans and Candida albicans are the main microorganisms that cause dental cavities. It can cause infection, damaged tissue around teeth, abscesses, and focal infection to other organs in the body. Natural products are currently widely used as products or as additives in the prevention of dental caries which have more anti-bacterial and anti-fungal activities than antibiotics that can cause resistance. Citronella (Cymbopogon citratus) is abundant and easy to grow. This study was conducted to determine the effectiveness of citronella (Cymbopogon citratus) oil on the growth of S. mutans and C. albicans microorganisms by Minimum Inhibitory Content (MIC) and Minimum Bactericidal Content (MBC). The method of MIC is broth microdilution by making chlorhexidine concentration levels of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, and 0.2% with 4 replications each. The MIC value was determined based on absorbance spectrophotometry and the MBC value was determined from the agar plate using the spread method. Biofilm eradication test was conducted by crystal-violet staining and measuring the absorbance. The results of MIC and MBC were obtained on S. mutans, namely at concentrations of 25% and 100%, respectively. Meanwhile, the results of MIC and MBC on C. albicans were obtained at concentrations of 50% and 100%, respectively. Furthermore, the citronella oil has antibacterial and antifungal activities.


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Hamada S and Slade HD. Biology, Immunology, and Cariogenicity of Streptococcus mutans. Microbiological Reviews. 1980; 44(2): 331-384.

Matsumoto-Nakano M. Role of Streptococcus Mutans Surface Proteins for Biofilm Formation. Japanese Dental Science Review. 2018; 54(1): 22-29.

Du Q, Ren B, He J, et al. Candida albicans Promotes Tooth Decay by Inducing Oral Microbial Dysbiosis. International Society for Microbial Ecology. 2021; 15(3): 894-908.

Falsetta ML, Klein MI, Colonne PM, et al. Symbiotic Relationship between Streptococcus Mutans and Candida Albicans Synergizes Virulence of Plaque Biofilms In Vivo. Infection and Immunity. 2014; 82(5): 1968-1981.

Quinto EJ, Caro I, Villalobos-Delgado LH, Mateo J, De-Mateo-Silleras B, and Redondo-Del-Río MP. Food Safety through Natural Antimicrobials. Antibiotics. 2019; 8(4): 1-30.

Munekata PES, Rocchetti G, Pateiro M, Lucini L, Domínguez R, and Lorenzo JM. Addition of Plant Extracts to Meat and Meat Products to Extend Shelf-Life and Health-Promoting Attributes: An Overview. Current Opinion in Food Science. 2020; 31: 81-87.

Ekpenyong CE and Akpan EE. Use of Cymbopogon Citratus Essential Oil in Food Preservation: Recent Advances and Future Perspectives. Critical Reviews in Food Science and Nutrition. 2017; 57(12): 2541-2559.

Dhakad AK, Pandey VV, Beg S, Rawat JM, and Singh A. Biological, Medicinal and Toxicological Significance of Eucalyptus Leaf Essential Oil: A Review. Journal of the Science of Food and Agriculture. 2018; 98(3): 833-848.

Victor IA, Adegoke AM, Olugbami JO, Gbadegesin MA, and Odunola OA. Lead-Induced Toxicities in Wistar Rats: Mitigating Effects of Ethanol Leaf Extract of Cymbopogon Citratus Stapf. Archives of Basic and Applied Medicine. 2020; 8(2): 123-129.

Lawal OA, Ogundajo AL, Avoseh NO, and Ogunwande IA. Cymbopogon Citratus. Medicinal Spices and Vegetables from Africa. Dschang, Cameroon: Academic Press; 2017; pp. 397-423.

Chaves-Quirós C, Usuga-Usuga JS, Morales-Uchima SM, Tofiño-Rivera AP, Tobón-Arroyave SI, Martínez-Pabón MC. Assessment of Cytotoxic and Antimicrobial Activities of Two Components of Cymbopogon Citratus Essential Oil. Journal of Clinical and Experimental Dentistry. 2020; 12(8): e749-e754.

Shah G, Shri R, Panchal V, Sharma N, Singh B, Mann AS. Scientific Basis for the Therapeutic Use of Cymbopogon citratus, Stapf (Lemon grass). Journal of Advanced Pharmaceutical Technology & Research. 2011; 2(1): 3-8.

Ariyani F, Setiawan LE, and Soetaredjo FE. Ekstraksi Minyak Atsiri dari Tanaman Sereh dengan Menggunakan Pelarut Metanol, Aseton, dan N-Heksana. Widya Teknik. 2008; 7(2): 124-133.

Garg J, Manjunath S, Sinha S, Ghambhir S, Abbey P, and Jungio MP. Antimicrobial Activity of Chlorhexidine and Herbal Mouthwash Against the Adherence of Microorganism to Sutures After Periodontal Surgery: A Clinical Microbiological Study. Cureus. 2022; 14(12): 1-9.

Chitsazha R, Faramarzi M, Sadighi M, et al. Evaluation of Antibacterial Effect of Concentrated Growth Factor on Aggregatibacter Actinomycetemcomitans and Porphyromonas Gingivalis. Journal of Family Medicine and Primary Care. 2022; 11(6): 2865-2869.

Kowalska-Krochmal B and Dudek-Wicher R. The Minimum Inhibitory Concentration of Antibiotics: Methods, Interpretation, Clinical Relevance. Pathogens. 2021; 10(2): 1-21.

Parvekar P, Palaskar J, Metgud S, Maria R, and Dutta S. The Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of Silver Nanoparticles Against Staphylococcus Aureus. Biomaterial Investigations in Dentistry. 2020; 7(1): 105-109.

Rahayu YC, Setiawatie EM, Rahayu RP, et al. Effects of Cocoa Pod Husk Extract (Theobroma Cacao L.) on Alveolar Bone in Experimental Periodontitis Rats. Trends in Sciences. 2023; 20(6): 1-8.

Wang Q and Xie M. Antibacterial Activity and Mechanism of Luteolin on Staphylococcus aureus. Wei Sheng Wu Xue Bao: Acta Microbiologica Sinica. 2010; 50(9): 1180-1184.

Sari I, Misrahanum M, Faradilla M, Ayuningsih CM, and Maysarah H. Antibacterial Activity of Citronella Essential Oil from Cymbopogon nardus (L.) Rendle) Against Methicillin-Resistant Staphylococcus aureus. Indonesian Journal of Pharmaceutical and Clinical Research. 2022; 5(1): 16-22.

Nguyen TLA and Bhattacharya D. Antimicrobial Activity of Quercetin: An Approach to Its Mechanistic Principle. Molecules. 2022; 27(8): 1-13.

Wu T, He M, Zang X, et al. A Structure-Activity Relationship Study of Flavonoids as Inhibitors of E. Coli by Membrane Interaction Effect. Biochimica et Biophysica Acta (BBA)-Biomembranes. 2013; 1828(11): 2751-2756.

Br. Tarigan BMC, Lelyana S, and Sugiaman VK. Kadar Hambat Minimum dan Kadar Bunuh Minimum Ekstrak Etanol Daun Oregano terhadap Pertumbuhan Candida Albicans. JITEKGI: Jurnal Ilmiah dan Teknologi Kedokteran Gigi. 2021; 17(2): 55-62.

Rao A, Zhang Y, Muend S, and Rao R. Mechanism of Antifungal Activity of Terpenoid Phenols Resembles Calcium Stress and Inhibition of the TOR Pathway. Antimicrobial agents and chemotherapy. 2010; 54(12): 5062-5069.

Kamal HZA, Ismail TNNT, Arief EM, and Ponnuraj KT. Antimicrobial Activities of Citronella (Cymbopogon Nardus) Essential Oil Against Several Oral Pathogens and Its Volatile Compounds. Padjajaran Journal of Dentistry. 2020; 32(1): 1-7.



2024-02-29 — Updated on 2024-02-29



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