Effect of Chitosan on Histology of Reproductive Organs of Female Wistar Rats (Rattus norvegicus) Exposed to Acetate Lead
DOI:
https://doi.org/10.21776/ub.jkb.2019.030.04.5Keywords:
Antral follicles, endometrial arterioles, chitosan, lead, thickness of endometriumAbstract
Lead is one of the pollutants widely spread in the environment because it is not easily decomposed. Lead can affect system functions such as the ovary and endometrium. Lead can trigger oxidative stress by reducing antioxidant enzymes and increasing Reactive Oxygen Species (ROS). Lead can also reduce Follicle Stimulating Hormone (FSH) and Luteinizing Hormone (LH) levels by disturbing the hypothalamus. Chitosan is an antioxidant compound that can reduce the toxic effects of lead. The purpose of this study was to study the effects of chitosan administration on the diameter of antral follicles, the number of endometrial arterioles, and the thickness of endometrial rats after lead acetate exposure. This study was an experimental laboratory using a posttest-only control group design approach applied on 25 female rats aged 8 weeks old, body weight 125-175 grams. Lead and chitosan were given orally with a sonde. There were 5 groups, namely, negative control group (without any treatment), positive control group (lead 175mg/kg/BW), treatment group 1 (lead 175mg/kg/BW + chitosan 16mg/kg/BW, treatment group 2 (lead 175mg/kg/BW + chitosan 32mg/kg/BW), and treatment group 3 (lead 175mg/kg/BW + chitosan 64mg/kg/BW) for 30 days. The rats were sacrificed at proestrus phase, which was proven from vaginal swab. Observations were carried out using the Hematoxylin Eosin (HE) staining method. The observations were analyzed using One Way ANOVA and followed by Least Significant Differences (LSD) test. The results showed significant results (p-value <0.05). Chitosan can increase the diameter of the antral follicle, increase the number of endometrial arterioles, and increase the thickness of endometrial rats exposed by lead acetate.Downloads
References
Conant J and Fadem P. A Community Guide to Environmental Health. Berkeley, California; Hesperian; 2012: p. 368.
Flora SJS and Agrawal S. Chapter 31 - Arsenic, Cadmium, and Lead. In: Gupta RC (Ed). Reproductive and Developmental Toxicology 2nd edition. Canada: Elsevier; 2017: p. 537-566.
Nascimento CRB, Risso WE, and Martinez CBDR. Lead Accumulation and Metallothionein Content in Female Rats of Different Ages and Generations After Daily Intake of Pb-Contaminated Food. Environmental Toxicology and Pharmacology. 2016; 48: 272-277.
Gillis BS, Arbieva Z, and Gavin IM. Analysis of Lead Toxicity in Human Cells. BioMed Central Genomics. 2012; 13: 1-12.
Ayala A, Muñoz MF, and Argüelles S. Lipid Peroxidation: Production, Metabolism, and Signaling Mechanisms of Malondialdehyde and 4-Hydroxy-2-Nonenal. Oxidative Medicine and Cellular Longevity. 2014; 2014: 1-31.
Flora SJS, Pachauri V, and Saxena G. Arsenic, Cadmium, and Lead. In: Gupta RC (Ed). Reproductive and Developmental Toxicology. Canada: Elsevier; 2011: p. 415-438.
Linardou H, Kalogeras KT, Kronenwett R, et al. The Prognostic and Predictive Value of MRNA Expression of Vascular Endothelial Growth Factor Family Members in Breast Cancer: A Study in Primary Tumors of High-Risk Early Breast Cancer Patients Participating in a Randomized Hellenic Cooperative Oncology Group Trial. Breast Cancer Research. 2012; 14(6): 1-16.
Sanders T, Liu Y, Buchner V, and Tchounwou PB. Neurotoxic Effects and Biomarkers of Lead Exposure: A Review. Reviews on Environmental Health. 2009; 24(1): 15-46.
Zorana KG, Alica P, and Jasna J. Influence of Abatement of Lead Exposure in Croatia on Blood Lead and ALAD Activity. Environmental Science and Pollution Research International. 2016; 23(1): 898-907.
Carocci A, Catalano A, Lauria G, Sinicropi MS, and Genchi G. Lead Toxicity, Antioxidant Defense and Environment. Reviews of Environmental Contamination and Toxicology. 2016; 238: 45-47.
Dumitrescu E, Chiurciu V, Muselin F, Popescu R, Brezovan D, and CRISTINA RT. Effects of Long-Term Exposure of Female Rats to Low Levels of Lead: Ovary and Uterus Histological Architecture Changes. Turkish Journal of Biology. 2015; 39(2): 284-289.
Hsueh AJW, Kawamura K, Cheng Y, and Fauser BCJM. Intraovarian Control of Early Folliculogenesis. Endocrine Reviews. 2015; 36(1): 1-24.
Hamilton KJ, Hewitt SC, Arao Y, and Korach KS. Estrogen Hormone Biology. Current Topics in Developmental Biology. 2017; 125: 109-146.
Barrett KE, Barman SM, Boitano S, and Brooks HL. Ganong's Review of Medical Physiology. 24th edition. New York: Mc Graw HIll; 2012: p. 539-553.
Sadekova ON, Nikitina LA, Rashidov TN, et al. Luteal Phase Defect is Associated with Impaired VEGF MRNA Expression in the Secretory Phase Endometrium. Reproductive Biology. 2015; 15(1): 65-68.
Kanzaki H. Uterine Endometrial Function. New York: Springer; 2016: p. 1-155.
Mescher AL. Junqueira's Basic Histology: Text and Atlas. New York: McGraw-Hill Education; 2013.
Ivanovski M. The Role of Ultrasound in the Evaluation of Endometrial Receptivity Following Assisted Reproductive Treatments. (Online) 2012. https://www.intechopen.com/books/in-vitro-fertilization-innovative-clinical-and-laboratory-aspects/the-role-of-ultrasound-in-the-evaluation-of-endometrial-receptivity-following-assisted-reproductive-.
Gupta P, Chandra S, Kaushik A, and Jain PK. Evaluation of Uterine Biophysical Profile and to Assess its Role in Predicting Conception among Unexplained Primary Infertility Patients. Indian Journal of Community Health. 2014; 26(4): 401-405.
Wang Z, Yan Y, Yu X, Li W, Li B, and Qin C. Protective Effects of Chitosan and Its Water-Soluble Derivatives Against Lead-Induced Oxidative Stress in Mice. International Journal of Biological Macromolecules. 2016; 83: 442-449.
Rajalakshmi A, Krithiga N, and Jayachitra A. Antioxidant Activity of the Chitosan Extracted from Shrimp Exoskeleton. Middle-East Journal of Scientific Research. 2013; 16(10): 1446-1451.
Mahdy Samar M, El-Kalyoubi MH, Khalaf MM, and Abd El-Razik MM. Physicochemical, Functional, Antioxidant and Antibacterial Properties of Chitosan Extracted from Shrimp Wastes by Microwave Technique. Annals of Agricultural Sciences. 2013; 58(1): 33-41.
Ngo DH and Kim SK. Antioxidant Effects of Chitin, Chitosan, and Their Derivatives. Advances in Food and Nutrition Research. 2014; 73: 15-31.
Sari AI, Destriani SN, Soeharto S, and Raharjo B. Aktivitas Antioksidan Kitosan pada Ovarium Tikus Betina yang Terpapar Timbal ( Pb ) Asetat. 2018; 4(2): 80-86. JUDUL JURNAL??
Ahmed YF, Eldebaky H, Mahmoud KGM, and Nawito M. Effects of Lead Exposure on DNA Damage and Apoptosis in Reproductive and Vital Organs in Female Rabbits. Global Veterinaria. 2012; 9(4): 401-408.
Dhir V and Dhand P. Toxicological Approach in Chronic Exposure to Lead on Reproductive Functions in Female Rats (Rattus Norvegicus). Toxicology International. 2010; 17(1): 1-7.
Silva-Santos KC, Marinho LSR, Santos GMG, et al. Ovarian Follicle Reserve: Emerging Concepts and Applications. Animal Reproduction. 2013; 10(3): 180-186.
Sánchez F and Smitz J. Molecular Control of Oogenesis. Biochimica et Biophysica Acta. 2012; 1822(12): 1896-1912.
Sharma R, Qureshi N, Mogra S, and Panwar K. Lead Induced Infertility in Swiss Mice and Role of Antioxidants. Universal Journal of Environmental Research and Technology. 2012; 2(2): 72-82.
Niu J, Lin HZ, Jiang SG, et al. Comparison of Effect of Chitin, Chitosan, Chitosan Oligosaccharide and N-Acetyl-D-Glucosamine on Growth Performance, Antioxidant Defenses and Oxidative Stress Status of Penaeus Monodon. Aquaculture. 2013; 372-375: 1-8.
Riswanda T. Pemanfaatan Kitosan Udang Putih (Lithopannaeus Vannamei) sebagai Bioabsorben Logam Berat Timbal (Pb) pada Daging Kerang Tahu di Muara Sungai Gunung Anyar. LenteraBio: Berkala Ilmiah Biologi. 2014; 3(3): 266-271.
Nakade UP, Garg SK, Sharma A, et al. Lead-Induced Adverse Effects on the Reproductive System of Rats with Particular Reference to Histopathological Changes in Uterus. Indian Journal of Pharmacology. 2015; 47(1): 22-26.
Dumitrescu E, Cristina RT, and Muselin F. Turkish Journal of Biology Reproductive Biology Study of Dynamics of Female Sexual Hormones: A 12-Month Exposure to Lead Acetate Rat Model. Turkish Journal of Biology. 2014; 38: 581-585.
Chianeh YR and Rao P. Molecular and Hormonal Regulation of Angiogenesis in Proliferative Endometrium. International Journal of Research in Medical Science. 2014; 2(1): 1-9.
Girling JE and Rogers PAW. Regulation of Endometrial Vascular Remodelling: Role of the Vascular Endothelial Growth Factor Family and the Angiopoietin–TIE Signalling System. Reproduction. 2009; 138(6): 883-893.
Keman K. Patomekanisme Preeklampsia Terkini: Mengungkapkan Teori-Teori Terbaru tentang Patomekanisme Preeklampsia Dilengkapi dengan Deskripsi Biomokuler. Malang: Universitas Brawijaya Press; 2014.
Lee SR, Lim YM, Jeong KA, and Chung H. Office Hysteroscopic Findings of Congested Endometrial Spiral Arterioles as Numerous Tiny Black Turtuous Lesions. The Journal of Minimally Invasive Gynecology. 2017; 24(2): 191-192.
Vedy HI. Efektifitas Kitosan Sebagai Absorben Logam Berat pada Gambaran Anatomi Ginjal Mencit (Mus Musculus L) yang Dinduksi Plumbum Asetat. Medical Journal of Lampung University. 2015; 4(7): 77-80.
Diantini NSE. Pengaruh Paparan Timbal Asetate Peroral Terhadap Peningkatan Kadar Malondialdehyde (Mda) Uterus Dan Penurunan Tebal Endometrium Tikus Putih (Rattus Norvegicus) Galur Wistar. [Tesis]. Universitas Brawijaya, Malang. 2016.
Qureshi N and Sharma R. Lead Toxicity and Infertility in Female Swiss Mice: A Review. Journal of Chemical, Biological and Physical Sciences. 2012; 2(4): 1849-1861.
Maulana HI, Ulilalbab A, Priyanto AD, dan Estiasih T. Effervescent Rosela Ungu Mencegah Penurunan Nilai SOD dan Mencegah Nekrosis Hepar Tikus Wistar yang Diberi Minyak Jelantah. Jurnal Kedokteran Brawijaya. 2014; 28(2): 85-90.
Wardani G and Sudjarwo SA. In Vitro Antibacterial Activity of Chitosan Nanoparticles Against Mycobacterium Tuberculosis. Pharmacognosy Journal. 2018; 10(1): 162-166
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