Antioxidant and Anti-Hyaluronidase Activities of Dragon Fruit Peel Extract and Kaempferol-3-O-Rutinoside
Keywords:Aging, anti-hyaluronidase, dragon fruit peel extract, kaempferol, H2O2 scavenging
AbstractAging is a natural process in human life and is triggered by the presence of free radicals (ROS). The use of antioxidants from natural ingredients is one of the breakthroughs to overcome aging and counteract the harmful effects caused by the free radicals. This study aimed to determine and compare the antioxidant activity of H2O2 scavenging and hyaluronidase inhibition of red dragon fruit peel extract (DFPE) and kaempferol-3-o-rutinoside (KOR) compounds. Dragon fruit peel extract (DFPE) is obtained through extraction by maceration method using 70% ethanol solvent. The design of this study included antioxidant and anti-aging activity assay of EKBN and KOR at the series concentration of 15.63; 31.25; 62.50; 125; 250; 500 Âµg/mL through H2O2 scavenging, as well as the DFPE and KOR hyaluronidase inhibition assay at the series concentration of 5.21; 10.42; 20.83; 41.7; 83.33; 166.67 Âµg/mL. EKBN shows that the average activity of H2O2 scavenging is lower than KOR. In addition, the IC50 values of KOR for H2O2 scavenging is lower (351.46Â±2.30ug/mL) than DFPE (409.64Â±23.17ug/mL). While, KOR also has higher values of inhibitory activity than of the DFPE. However, the IC50 value of KOR for hyaluronidase inhibition activity was 84.07Â±10.46Âµg/mL, equivalent to the IC50 value of DFPE (85.32Â±10.24Âµg/mL). The presence of antioxidant and anti-aging activity in the EKBN is probably caused by betalain and the KOR compound itself contained in red dragon fruit. The results of the paired-samples T-test on antioxidant activity and anti-aging of DFPE and KOR showed non-significant difference. Thus, DFPE has an equivalent antioxidant and anti-aging through H2O2 scavenging and hyaluronidase activity as possessed by the KOR compound.
Poon F, Kang S, and Chien AL. Mechanisms and Treatments of Photoaging. Photodermatology, Photoimmunology & Photomedicine. 2015; 31(2): 65-74.
Sjerobabski-Masnec I and Å itum M. Skin Aging. Acta Clinica Croatica. 2010; 49(4): 515-518.
Berneburg M, Trelles M, Friguet B, et al. How Best to Halt and/or Revert UV-Induced Skin Ageing: Strategies, Facts and Fiction. Experimental Dermatology. 2008; 17(3): 228-240.
Gomes EC, Silva AN, and de Oliveira MR. Oxidants, Antioxidants, and the Beneficial Roles of Exercise-Induced Production of Reactive Species. Oxidative Medicine and Cellular Longevity. 2012; 2012: 1-12.
Widowati W, Noverina R, Ayuningtyas W, et al. Reactive Oxygen Species and Aging Mechanism. In: Wilkerson S (Ed). Reactive Oxygen Species (ROS) Mechanisms and Role in Health and Disease. New York: Nova Science Publishers; 2018: pp. 101-134.
Mc Auley MT, Guimera AM, Hodgson D, et al. Modelling the Molecular Mechanisms of Aging. Bioscience Reports. 2017; 37(1): 1-20.
Thring TS, Hili P, and Naughton DP. Anti-Collagenase, Anti-Elastase and Anti-Oxidant Activities of Extracts from 21 Plants. BioMed Central Complementary and Alternative Medicine. 2009; 9(1): 1-11.
Mukherjee PK, Maity N, Nema NK, and Sarkar BK. Bioactive Compounds from Natural Resources Against Skin Aging. Phytomedicine. 2011; 19(1): 64-73.
Jaafar RA, Rahman ARA, Mahmod NZC, and Vasudevan R. Proximate Analysis of Dragon Fruit (Hylecereus Polyhizus). American Journal of Applied Sciences. 2009; 6(7): 1341-1346.
Panjuantiningrum F. Pengaruh Pemberian Buah Naga Merah terhadap Kadar Glukosa Darah Tikus Putih yang Diinduksi Aloksan. [Skripsi]. Universitas Sebelas Maret, Semarang. 2009.
Calderon-Montano JM, Burgos-Moron E, Perez-Guerrero C, and Lopez-Lazaro M. A Review on the Dietary Flavonoid Kaempferol. Mini-Reviews in Medicinal Chemistry. 2011; 11(4): 298-344.
Widowati W, Fauziah N, Herdiman H, et al. Antioxidant and Anti Aging Assays of Oryza Sativa Extracts, Vanillin and Coumaric Acid. Journal of Natural Remedies. 2016; 16(3): 88-99.
Widowati W, Rani AP, Hamzah RA, et al. Antioxidant and Antiaging Assays of Hibiscus Sabdariffa Extract and Its Compounds. Natural Product Sciences. 2017; 23(3): 192-200.
Widowati W, Janeva WB, Nadya S, et al. Antioxidant and Antiaging Activities of Jasminum Sambac Extract, and Its Compounds. Journal of Reports in Pharmaceutical Sciences. 2018; 7(3): 270-285.
Mukhopadhyay D, Dasgupta P, Roy DS, et al. A Sensitive In Vitro Spectrophotometric Hydrogen Peroxide Scavenging Assay Using 1,10-Phenanthroline. Free Radicals and Antioxidants. 2016; 6(1): 124-132.
Tu P and Tawata S. Anti-Oxidant, Anti-Aging, and Anti-Melanogenic Properties of the Essential Oils from Two Varieties of Alpinia Zerumbet. Molecules. 2015; 20(9): 16723-16740.
Evacuasiany E, Ratnawati H, Liana L, et al. Cytotoxic and Antioxidant Activities of Catechins in Inhibiting the Malignancy of Breast Cancer. Oxidants and Antioxidants in Medical Science. 2014; 3(2): 141-146.
Lourith N and Kanlayavattanakul M. Antioxidant and Stability of Dragon Fruit Peel Colour. Agro FOOD Industry Hi Tech. 2013; 24(3): 56-58.
Suh DH, Lee S, Heo DY, et al. Metabolite Profiling of Red and White Pitayas ( Hylocereus Polyrhizus and Hylocereus Undatus ) for Comparing Betalain Biosynthesis and Antioxidant Activity. Journal of Agricultural and Food Chemistry. 2014; 62(34): 8764-8771.
Badal S and Delgoda R. Pharmacognosy Fundamentals, Applications, and Strategies. 1st edition. Amsterdam: Elsevier; 2017.
R Nurliyana, Zahir IS, Suleiman KM, Aisyah M, and Rahim KK. Antioxidant Study of Pulps and Peels of Dragon Fruits: A Comparative Study. International Food Research Journal. 2010; 17: 367-375.
Tatsimo SJN, Tamokou JD, Havyarimana L, et al. Antimicrobial and Antioxidant Activity of Kaempferol Rhamnoside Derivatives from Bryophyllum Pinnatum. BioMed Central Research Notes. 2012; 5(1): 1-6.
Vellosa JCR, Regasini LO, Khalil NM, et al. Antioxidant and Cytotoxic Studies for Kaempferol, Quercetin and Isoquercitrin. EclÃ©tica QuÃmica. 2011; 36(2): 7-20.
Nasution AS, Wirjatmadi B, dan Adriani M. Efek Preventif Pemberian Ekstrak Kulit Buah Naga Berdaging Super Merah (Hylocereus Costaricensis) terhadap Malondialdehid Tikus Wistar yang Dipapar Asap Rokok. Jurnal Kedokteran Brawijaya. 2016; 29(1): 21-24.
Nema NK, Maity N, Sarkar BK, and Mukherjee PK. Matrix Metalloproteinase, Hyaluronidase and Elastase Inhibitory Potential of Standardized Extract of Centella Asiatica. Pharmaceutical Biology. 2013; 51(9): 1182-1187.
Papakonstantinou E, Roth M, and Karakiulakis G. Hyaluronic Acid: A Key Molecule in Skin Aging. Dermato-Endocrinology. 2012; 4(3): 253-258.
Antognoni F, Perellino NC, Crippa S, et al. Irbic Acid, A Dicaffeoylquinic Acid Derivative from Centella Asiatica Cell Cultures. Fitoterapia. 2011; 82(7): 950-954.
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