Comparison of Antioxidant and Anti-Tyrosinase Activities of Pineapple (Ananas comosus) Core Extract and Luteolin Compound
Keywords:Aging, DPPH free radical, luteolin compounds, pineapple core extract, tyrosinase enzyme
AbstractFree radicals and UV exposure can cause aging. Aging prevention needs substances that can prevent molecular oxidation reactions in cells and inhibit the activity of enzymes that trigger aging. Research on pineapple skin and flesh extract (Ananas comosus (L.) Merr.) reported the presence of luteolin compound which functions as antioxidants and antityrosinase. However, in this study, the object used was pineapple core extract (PCE), which has not been widely known for its antioxidant and antityrosinase activity. Therefore, the purpose of this study was to determine the content of phytochemical compounds, antioxidant activity, and inhibitory activity of tyrosinase enzymes by PCE and then compared with the luteolin (LT) compound test results using Fansworth method, DPPH scavenging activity assay and tyrosinase enzyme inhibition assay. Based on the study results, the phytochemical compounds contained in PCE were tannins and triterpenoids.Â PCE and LT produced the highest DPPH scavenging activity, which was 64.86% and 59.32% (final concentration 200Î¼g/ml and 6.25Î¼g/ml) and the highest tyrosinase inhibition activity, which was 60.52% and 85.02% (final concentration 100 Î¼g/ml). Antioxidant activity was determined based on IC50 of 87.46Âµg/mL and 4.17 Âµg/ml respectively. IC50 tyrosinase enzyme inhibition EBN and LT respectively at 62.27Âµg/ml and 5.25 Âµg/ml. Antioxidant activity through DPPH free radical scavenging test and tyrosinase enzyme inhibition activity by PCE was lower than LT.
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