Comparison of Antioxidant and Anti-Tyrosinase Activities of Pineapple (Ananas comosus) Core Extract and Luteolin Compound


  • Dela Vrianty Universitas Prima Indonesia
  • Rismawati Laila Qodariah Aretha Medika Utama
  • Wahyu Widowati Universitas Kristen Maranatha
  • Ade Putra Fratama Sinaga Universitas Prima Indonesia
  • Dewi Fibrina Universitas Prima Indonesia
  • Edy Fachrial Universitas Prima Indonesia
  • I Nyoman Ehrich Lister Universitas Prima Indonesia



Aging, DPPH free radical, luteolin compounds, pineapple core extract, tyrosinase enzyme


Free radicals and UV exposure can cause aging. Aging prevention needs substances that can prevent molecular oxidation reactions in cells and inhibit the activity of enzymes that trigger aging. Research on pineapple skin and flesh extract (Ananas comosus (L.) Merr.) reported the presence of luteolin compound which functions as antioxidants and antityrosinase. However, in this study, the object used was pineapple core extract (PCE), which has not been widely known for its antioxidant and antityrosinase activity. Therefore, the purpose of this study was to determine the content of phytochemical compounds, antioxidant activity, and inhibitory activity of tyrosinase enzymes by PCE and then compared with the luteolin (LT) compound test results using Fansworth method, DPPH scavenging activity assay and tyrosinase enzyme inhibition assay. Based on the study results, the phytochemical compounds contained in PCE were tannins and triterpenoids.  PCE and LT produced the highest DPPH scavenging activity, which was 64.86% and 59.32% (final concentration 200μg/ml and 6.25μg/ml) and the highest tyrosinase inhibition activity, which was 60.52% and 85.02% (final concentration 100 μg/ml). Antioxidant activity was determined based on IC50 of 87.46µg/mL and 4.17 µg/ml respectively. IC50 tyrosinase enzyme inhibition EBN and LT respectively at 62.27µg/ml and 5.25 µg/ml. Antioxidant activity through DPPH free radical scavenging test and tyrosinase enzyme inhibition activity by PCE was lower than LT.


Download data is not yet available.


Nur S, Rumiyati R, and Lukitaningsih E. Screening of Antioxidants, Anti-Aging and Tyrosinase Inhibitory Activities of Ethanolic and Ethyl Acetate Extracts of Fruit Flesh and Fruit Peel Langsat (Lansium domesticum Corr) In Vitro. Majalah Obat Tradisional. 2017; 22(1): 63-72.

Widowati W, Noverina R, Ayuningtyas W, et al. Reactive Oxygen Species and Aging Mechanism. In: Wilkerson S (Ed). Reactive Oxygen Species (ROS) Mechanisms and Role in Health and Disease. New York: Nova Science Publishers; 2018: pp. 101-134.

Halliwell B and Gutteridge JMC. Free radicals in biology and medicine. New York: Oxford University Press; 2015.

Widowati W, Fauziah N, Herdiman H, et al. Antioxidant and Anti Aging Assays of Oryza Sativa Extracts, Vanillin and Coumaric Acid. Journal of Natural Remedies. 2016; 16(3): 88-99.

Rusmana D, Wahyudianingsih R, Elisabeth M, Balqis, Maesaroh, and Widowati W. Antioxidant Activity of Phyllanthus niruri Extract, Rutin and Quercetin. The Indonesian Biomedical Journal. 2017; 9(2): 84-90.

Tanigawa T, Kanazawa S, Ichibori R, et al. (+)-Catechin Protects Dermal Fibroblasts Against Oxidative Stress-Induced Apoptosis. BioMed Central Complementary and Alternative Medicine. 2014; 14(1): 1-7.

Fidrianny I, Virna V, and Insanu M. Antioxidant Potential of Different Parts of Bogor Pineapple (Ananas comosus [L.] Merr. Var. Queen) Cultivated in West Java-Indonesia. Asian Journal of Pharmaceutical and Clinical Research. 2018; 11(1): 129-133.

Adhikarimayum H, Kshetrimayum G, and Maibam D. Evaluation of Antioxidant Properties of Phenolics Extracted from Ananas comosus L. Notulae Scientia Biologicae. 2010; 2(2): 68-71.

da Silva DIS, Nogueira GD, Duzzioni AG, and Barrozo MAS. Changes of Antioxidant Constituents in Pineapple (Ananas comosus) Residue during Drying Process. Industrial Crops and Products. 2013; 50: 557-562.

Hossain MA and Rahman SM. Total Phenolics, Flavonoids, and Antioxidant Activity of Tropical Fruit Pineapple. Food Research International. 2011; 44(3): 672-676.

An SM, Kim HJ, Kim JE, and Boo YC. Flavonoids, Taxifolin and Luteolin Attenuate Cellular Melanogenesis Despite Increasing Tyrosinase Protein Levels. Phytotherapy Research. 2008; 22(9): 1200-1207.

Lin Y, Shi R, Wang X, and Shen HM. Luteolin, a Flavonoid with Potential For Cancer Prevention And Therapy. Current Cancer Drug Targets. 2008; 8(7): 634-646.

Upadhyay A, Lama JP, and Tawata S. Utilization of Pineapple Waste: A Review. Journal of Food Science and Technology Nepal. 2010; 6: 10-18.

Widowati W, Rani AP, Hamzah RA, et al. Antioxidant and Antiaging Assays of Hibiscus Sabdariffa Extract and Its Compounds. Natural Product Sciences. 2017; 23(3): 192-200.

Widowati W, Janeva WB, Nadya S, et al. Antioxidant and Antiaging Activities of Jasminum Sambac Extract, and Its Compounds. Journal of Reports in Pharmaceutical Sciences. 2018; 7(3): 270-285.

Tu P and Tawata S. Anti-Oxidant, Anti-Aging, and Anti-Melanogenic Properties of The Essential Oils From Two Varieties of Alpinia Zerumbet. Molecules. 2015; 20(9): 16723-16740.

Asthana S, Zucca P, Vargiu A V, Sanjust E, Ruggerone P, and Rescigno A. Structure-Activity Relationship Study of Hydroxycoumarins and Mushroom Tyrosinase. Journal of Agricultural and Food Chemistry. 2015; 63(32): 7236-7244.

Bera TK, Chatterjee K, and Ghosh D. In-Vitro Antioxidant Properties of the Hydro-Methanol Extract of the Seeds of Swietenia Mahagoni (L.) Jacq. Biomarkers and Genomic Medicine. 2015; 7(1): 18-24.

Kalaskar MG and Surana SJ. Free Radical Scavenging and Hepatoprotective Potential of Ficus Microcarpa L. Fil. Bark Extracts. Journal of Natural Medicines. 2011; 65(3-4): 633-640.

Karim AA, Azlan A, Ismail A, et al. Phenolic Composition, Antioxidant, Anti-Wrinkles and Tyrosinase Inhibitory Activities of Cocoa Pod Extract. BioMed Central Complementary and Alternative Medicine. 2014; 14(381): 1-13.

Pouillot A, Polla LL, Tacchini P, Neequaye A, Polla A, and Polla B. Natural Antioxidants and Their Effects on the Skin. In: Dayan N and Kromidas L (Eds). Formulating, Packaging, and Marketing of Natural Cosmetic Products [Internet]. Hoboken: John Wiley & Sons, Inc.; 2011: p. 239-257.

Evacuasiany E, Ratnawati H, Liana L, et al. Cytotoxic and Antioxidant Activities of Catechins in Inhibiting the Malignancy of Breast Cancer. Oxidants and Antioxidants in Medical Science. 2014; 3(2): 141-146.

Widowati W, Herlina T, Ratnawati H, Constantia G, Deva IDGS, and Maesaroh. Antioxidant Potential of Black, Green and Oolong Tea Methanol Extracts. Biology, Medicine, & Natural Product Chemistry. 2015; 4(2): 35-39.

Cysilia K Hindarto, Endang Sri Lestari, Candra Irawan HR, and Rochaeni H. Antioxidant Activity of Luteolin Extracted from Nutshell Waste Arachis Hypogea. International Journal of Research in Pharmacy and Pharmaceutical Sciences. 2017; 2(6): 28-30.

Carniol PJ, Woolery-Lloyd H, Zhao AS, and Murray K. Laser Treatment For Ethnic Skin. Facial Plastic Surgery Clinics of North America. 2010; 18(1): 105-110.

Ebanks J, Wickett R, and Boissy R. Mechanisms Regulating Skin Pigmentation: The Rise and Fall of Complexion Coloration. International Journal of Molecular Sciences. 2009; 10(9): 4066-4087.

Chang TS. An Updated Review of Tyrosinase Inhibitors. International Journal of Molecular Sciences. 2009; 10(6): 2440-2475.

Gazali M, Zamani NP, and Batubara I. Potensi Limbah Kulit Buah Nyirih Xylocarpus Granatum sebagai Inhibitor Tirosinase. Depik. 2014; 3(3): 187-194.

Park JW, Ha YM, Moon K, et al. De Novo Tyrosinase Inhibitor: 4-(6,7-Dihydro-5H-Indeno[5,6-D]Thiazol-2-Yl)Benzene-1,3-Diol (MHY1556). Bioorganic & Medicinal Chemistry Letters. 2013; 23(14): 4172-4176.

Ha YM, Park YJ, Kim JA, et al. Design and Synthesis of 5-(Substituted Benzylidene) Thiazolidine-2,4-Dione Derivatives as Novel Tyrosinase Inhibitors. European Journal of Medicinal Chemistry [Internet]. 2012; 49: 245-252






Research Article

Most read articles by the same author(s)