Pengaruh Pemberian Ekstrak Propolis terhadap Ekspresi Bcl2 dan Apoptosis pada Sel Otak Tikus Model Cedera Otak Traumatik

Authors

  • Ully Husna Laboratorium Ilmu Penyakit Saraf Rumah Sakit Umum Daerah Dr. Saiful Anwar Malang Alamat : Jl. Jaksa Agung Suprapto 2 Malang, Jawa Timur No Telp : (62) (0341) 321297 Email : neuromalang.fk@ub.ac.id; ullyhusna@yahoo.com
  • Hidayat Sujuti Laboratorium Biomedik Fakultas Kedokteran Universitas Brawijaya Alamat : Jl. Veteran Malang-65145, Jawa Timur No. Telp : (62) (0341) 569117, 567192 Email : biomedik.fkub@gmail.com; hidayatsujuti@yahoo.com
  • Mochammad Dalhar Laboratorium Ilmu Penyakit Saraf Rumah Sakit Umum Daerah Dr. Saiful Anwar Malang Alamat :Jl. Jaksa Agung Suprapto 2 Malang, Jawa Timur No. Telp : (62) (0341) 321297 Email : neuromalang.fk@ub.ac.id; dalhar@yahoo.com

DOI:

https://doi.org/10.21776/ub.jkb.2017.029.03.3

Keywords:

Apoptosis sel, cedera otak traumatik, ekspresi Bcl-2, ekstrak propolis

Abstract

Ekstrak propolis mempunyai efek neuroprotektan melalui berbagai macam cara kerja salah satunya sebagai antioksidan karena bahan kandungan utamanya adalah flavonoid. Penelitian ini bertujuan untuk menguji efek ekstrak propolis berbagai dosis dalam meningkatkan ekspresi Bcl-2 dan menurunkan apoptosis sel otak tikus model cedera otak traumatik. Tikus dibagi menjadi 5 kelompok, yaitu: kelompok normal, kelompok yang diberi perlakuan cedera otak traumatis, kelompok yang diberi perlakuan cedera otak traumatis dan ekstrak propolis masing-masing dosis 50mg/kgBB, 100mg/kgBB, dan 200mg/kgBB. Setiap kelompok diambil otaknya untuk diperiksa ekspresi Bcl-2 dan apoptosis sel otak pada hari ke-7. Berdasarkan hasil analisa statistik, didapatkan hubungan signifikan antara ekspresi Bcl-2 dan apoptosis sel otak tikus model cedera otak traumatik dengan berbagai dosis ekstrak propolis (p<0,05). Dosis 200mg/kgbb merupakan dosis yang paling efektif dalam meningkatkan ekspresi Bcl2 otak tikus dan menurunkan apoptosis sel otak tikus. Penelitian ini telah membuktikan bahwa ekstrak propolis dapat meningkatkan ekspresi Bcl-2 dan menurunkan apoptosis sel otak tikus model cedera otak traumatik.

Kata Kunci: Apoptosis sel, cedera otak traumatik, ekspresi Bcl-2, ekstrak propolis

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Published

2017-01-10

Issue

Vol 29, No. 3 (2017)

Section

Research Article

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