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Distribusi M. Tuberculosis Genotipe Beijing pada Pasien Tuberkulosis Paru di Malang

Tri Yudani MR, Triwahju Astuti
  Jurnal Kedokteran Brawijaya, Vol 26, No 2 (2010),  pp.75-84  

Abstract


ABSTRAK
Penelusuran  penyebaran  genetis  Mycobacterium  tuberculosis  merupakan  salah  satu  faktor  penting  dalam
mengendalikan infeksi Mycobacterium tuberculosis. Studi mengenai distribusi Mycobacterium tuberculosis galur Beijing
pada TB paru BTA (+) pasien di RSU dr. Saiful Anwar rumah sakit ini   dilaksanakan dengan metode spoligotyping. Dahak
dikumpulkan dari total 41 pasien, dicuci dengan salin normal dan ditanam pada medium LJ. DNA dari bakteri yang tumbuh
kemudian diisolasi dan mengalami spoligotyping. Sembilan belas spoligotype (65,5%) ditemukan dari total dua puluh
sembilan sampel yang berkembang, sedangkan sepuluh sampel (34,5%) adalah non-Mycobacterium tuberculosis. Dari
spoligotype sembilan belas, sembilan sampel merupakan Beijing strain (31%) dan sepuluh sampel (34,5%) menunjukkan
strain non Beijing. Proporsi pasien TB yang terinfeksi, menunjukkan bahwa persentase pasien terinfeksi dengan strain
Beijing hampir sama antara wanita dan pria. Ditemukan bahwa prevalensi tertinggi strain Beijing pada pasien dengan usia
berkisar antara 31-40 tahun (51%). Oleh karena itu dapat disimpulkan bahwa strain Beijing mendominasi distribusi M.
tuberculosis pada pasien TB paru di Malang.
Kata Kunci: Beijing strain, M. tuberculosis, Spoligotyping

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References


DAFTAR PUSTAKA

Wird IRIN. Indonesia: Overcrowding fuels TB in prisons. (Online) 2010. http//www.IRINnews.org [diakses tanggal 5 September 2008].

Kamerbeek J, Scouls L, Kolk A, et al. Simultaneous Detection and Strain Differentiation of Mycobacterium tuberculosis for Diagnosis Epidemiology. Journal of Clinical Microbiology. 1997;35: 907-914.

Caminero JA, Pena MJ, Campos-Herrero MI, et al. Epidemiological Evidence of the Spread of a Mycobacterium tuberculosis Strain of the Beijing Genotype on Gran Canaria Island. American Journal of Respiratory and Critical Care Medicine. 2001; 164:1165-1170.

Van Soolingen D. Molecular Epidemiology of Tuberculosis and Other Mycobacterial Infections:Main Methodologies and Achievements. Journal of Internal Medicine.2001;249:1–26.

Parwati I, Van Crevel R, Sudiro M, et al. Mycobacterium tuberculosis Population Structures Differ Significantly on Two Indonesian Islands. Journal Clinical Microbiology. 2008; 46(11):3639-3654.

Niemann S, Reusch-Gerdes S, and Richter E. IS6110 Fingerprinting of Drug-resistant Mycobacterium tuberculosis Strains Isolated in Germany during 1995. Journal Clinical Microbiology.1997; 35(12): 3015-3020.

Diaz R, Kremer K, de Haas PE, et al. Molecular Epidemiologyof Tuberculosis in Cuba Outside of Havana. July 1994-June 1995:Utility of Spoligotyping Versus IS6110 Restriction Fragment Length of Polymorphism. International Journal Tuberculosis Lung Disease. 1998; 2(9): 668-743.

Krummer AS, Hoffner E, Sillastu H, et al. Spread of Drug Resistant Pulmonary Tuberculosis in Estoania.Journal Clinical Mocrobiology. 2001; 39(9): 3339-35: 907-914.3345.

Narvskaya O, Mokrousow L, Limeschenko E, et al. Molecular Characterization of Mycobacterium tuberculosis Strains from the Northwest Region of Russia. (Online.) http://www.epinorth.org/english/2000/2/002c.shtml[diakses tanggal 5 September 2008].

Toungoussova OS, Sandven P, Mariandyshev AO, Nizovtseva NI, Bjune G, and Cougant DA. Spread of Drug-resistant Mycobacterium tuberculosis Strains of the Beijing Genotype in the Archangel Oblast, Russia. Journal of Clinical Microbiology. 2002; 40(6): 1930-1937.

Agerton TB, Valway SE, Blinkbora RJ, et al. Origin and Interstate Spread of a New Yoork City Multidrug-resistant Mycobacterium tuberculosis Clone The Journal of The American Medical Association. 1996 ; 275(6): 452-457.

Bifani PJ, Mathema B, Liu Z, et al. Identification of a W Variant Outbreak of Mycobacterium tuberculosis f via Population-based Molecular Epidemiology. The Journal of The American Medical Association. 1996; 75(6): 2321-2327.

Van der Zanden A. Spoligotyping, a Tool in Epidemiology, Diagnosis and Control of Tuberculosis. [Thesis]. Bilthoven, The Netherlands. 2002.

Groenen PMA, Buncschoten AE, van Soolingen D and van Embden JDA. Nature of DNA Polymorphism in the Direct Repeat Cluster of Mycobacterium tuberculosis; Application for Strain Differentiation a Novel Method. Molecural Microbiology. 1993; 10(5): 1057-1065.

Brudey K, Driscoll JR, Rigouts L, et al. Mycobacterium

tuberculosis Complex Genetic Diversity: Mining the Fourth International Spoligotyping Database (SpolDB4) for Classification, Population Genetics and Epidemiology. BioMed Central Microbiology. 2006; 6: 23.




DOI: http://dx.doi.org/10.21776/ub.jkb.2010.026.02.3

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